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rabbit anti il33  (Proteintech)


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    Structured Review

    Proteintech rabbit anti il33
    Rabbit Anti Il33, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+il33/IL-33+Antibody/pmc11461611-249-56-58
    Average 94 stars, based on 57 article reviews
    rabbit anti il33 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors.
    Article Snippet: Progesterone (S1705), Nitazoxanide (S1627), Leflunomide (S1247), Nitrendipine (S2491), Lomerizine 2HCl (S4084), Econazole Nitrate (S2535), Hexachlorophene (S4632), Hydroxyprogesterone caproate (S4674), Dichlorophen (S5724) all purchased from Selleck, Ulipristal acetate (MEC, HY-N0437) and Progesterone analogs all purchased from MCE. .. The antibodies used in this study were obtained from the indicated source: rabbit anti-HA(CST, 3724; WB, 1:5,000), mouse anti-FLAG (Sigma, F3165; WB, 1:5,000), mouse anti-β-tubulin (ZENBIO, 200608; WB, 1:5,000), mouse anti-Myc (CST, 2276; WB, 1:5,000; IF, 1:500), goat anti-IL1β (R&D Systems, BAF401; WB, 1:2,000), rabbit anti-IL1β (Abcam, ab9722; WB, 1:5,000), rabbit anti-GSDMD (CST, 39754; WB: 1:2,000), rabbit anti-IL33 (Proteintech, 12372-1-AP; WB, 1:1,000), rabbit anti-V5 (CST, 13202; WB, 1:1,000; IF, 1:500), mouse anti-V5 (CST, 80076; WB, 1:5,000), rabbit anti-TMED10 (Proteintech, 15199-1-AP; WB, 1:3,000), rabbit anti-TMED2 (Proteintech, 11981-1-AP; WB, 1:3,000), mouse antiGST (CST, 2624; WB, 1:5,000), rabbit anti-ERGIC53 (Sigma, E1031; WB, 1:2,000), rabbit anti-GFP (CST, 2956; WB, 1:5,000), rabbit anti-Caspase3 (CST, 9662; WB: 1:2,000), rabbit anti-RPN1 (raised against C-terminal peptides corresponding to human protein residues 588–605 and mouse protein residues 576–605; from R. Schekman; WB, 1:5,000) and rabbit anti-E-SARS2 (raised against C-terminal peptides corresponding to the last 25 residues of E-SARS2 protein; purified by ABclonal; WB, 1:1,000). .. Goat antirabbit IgG Alexa Fluor 568 (Invitrogen, A-11011), goat anti-mouse IgG Alexa Fluor 568 (Invitrogen, A-11004) and goat anti-mouse IgG Alexa Fluor Plus 647 (Invitrogen, A32733) were used at a dilution of 1:500 for IF.

    Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors
    Article Snippet: Progesterone (S1705), Nitazoxanide (S1627), Leflunomide (S1247), Nitrendipine (S2491), Lomerizine 2HCl (S4084), Econazole Nitrate (S2535), Hexachlorophene (S4632), Hydroxyprogesterone caproate (S4674), Dichlorophen (S5724) all purchased from Selleck, Ulipristal acetate (MEC, HY-N0437) and Progesterone analogs all purchased from MCE. .. The antibodies used in this study were obtained from the indicated source: rabbit anti-HA(CST, 3724; WB, 1:5,000), mouse anti-FLAG (Sigma, F3165; WB, 1:5,000), mouse anti-β-tubulin (ZENBIO, 200608; WB, 1:5,000), mouse anti-Myc (CST, 2276; WB, 1:5,000; IF, 1:500), goat anti-IL1β (R&D Systems, BAF401; WB, 1:2,000), rabbit anti-IL1β (Abcam, ab9722; WB, 1:5,000), rabbit anti-GSDMD (CST, 39754; WB: 1:2,000), rabbit anti-IL33 (Proteintech, 12372-1-AP; WB, 1:1,000), rabbit anti-V5 (CST, 13202; WB, 1:1,000; IF, 1:500), mouse anti-V5 (CST, 80076; WB, 1:5,000), rabbit anti-TMED10 (Proteintech, 15199-1-AP; WB, 1:3,000), rabbit anti-TMED2 (Proteintech, 11981-1-AP; WB, 1:3,000), mouse anti-GST (CST, 2624; WB, 1:5,000), rabbit anti-ERGIC53 (Sigma, E1031; WB, 1:2,000), rabbit anti-GFP (CST, 2956; WB, 1:5,000), rabbit anti-Caspase3 (CST, 9662; WB: 1:2,000), rabbit anti-RPN1 (raised against C-terminal peptides corresponding to human protein residues 588–605 and mouse protein residues 576–605; from R. Schekman; WB, 1:5,000) and rabbit anti-E-SARS2 (raised against C-terminal peptides corresponding to the last 25 residues of E-SARS2 protein; purified by ABclonal; WB, 1:1,000). .. Goat anti-rabbit IgG Alexa Fluor 568 (Invitrogen, A-11011), goat anti-mouse IgG Alexa Fluor 568 (Invitrogen, A-11004) and goat anti-mouse IgG Alexa Fluor Plus 647 (Invitrogen, A32733) were used at a dilution of 1:500 for IF.

    Purification:

    Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors.
    Article Snippet: Progesterone (S1705), Nitazoxanide (S1627), Leflunomide (S1247), Nitrendipine (S2491), Lomerizine 2HCl (S4084), Econazole Nitrate (S2535), Hexachlorophene (S4632), Hydroxyprogesterone caproate (S4674), Dichlorophen (S5724) all purchased from Selleck, Ulipristal acetate (MEC, HY-N0437) and Progesterone analogs all purchased from MCE. .. The antibodies used in this study were obtained from the indicated source: rabbit anti-HA(CST, 3724; WB, 1:5,000), mouse anti-FLAG (Sigma, F3165; WB, 1:5,000), mouse anti-β-tubulin (ZENBIO, 200608; WB, 1:5,000), mouse anti-Myc (CST, 2276; WB, 1:5,000; IF, 1:500), goat anti-IL1β (R&D Systems, BAF401; WB, 1:2,000), rabbit anti-IL1β (Abcam, ab9722; WB, 1:5,000), rabbit anti-GSDMD (CST, 39754; WB: 1:2,000), rabbit anti-IL33 (Proteintech, 12372-1-AP; WB, 1:1,000), rabbit anti-V5 (CST, 13202; WB, 1:1,000; IF, 1:500), mouse anti-V5 (CST, 80076; WB, 1:5,000), rabbit anti-TMED10 (Proteintech, 15199-1-AP; WB, 1:3,000), rabbit anti-TMED2 (Proteintech, 11981-1-AP; WB, 1:3,000), mouse antiGST (CST, 2624; WB, 1:5,000), rabbit anti-ERGIC53 (Sigma, E1031; WB, 1:2,000), rabbit anti-GFP (CST, 2956; WB, 1:5,000), rabbit anti-Caspase3 (CST, 9662; WB: 1:2,000), rabbit anti-RPN1 (raised against C-terminal peptides corresponding to human protein residues 588–605 and mouse protein residues 576–605; from R. Schekman; WB, 1:5,000) and rabbit anti-E-SARS2 (raised against C-terminal peptides corresponding to the last 25 residues of E-SARS2 protein; purified by ABclonal; WB, 1:1,000). .. Goat antirabbit IgG Alexa Fluor 568 (Invitrogen, A-11011), goat anti-mouse IgG Alexa Fluor 568 (Invitrogen, A-11004) and goat anti-mouse IgG Alexa Fluor Plus 647 (Invitrogen, A32733) were used at a dilution of 1:500 for IF.

    Article Title: Coronavirus envelope protein activates TMED10-mediated unconventional secretion of inflammatory factors
    Article Snippet: Progesterone (S1705), Nitazoxanide (S1627), Leflunomide (S1247), Nitrendipine (S2491), Lomerizine 2HCl (S4084), Econazole Nitrate (S2535), Hexachlorophene (S4632), Hydroxyprogesterone caproate (S4674), Dichlorophen (S5724) all purchased from Selleck, Ulipristal acetate (MEC, HY-N0437) and Progesterone analogs all purchased from MCE. .. The antibodies used in this study were obtained from the indicated source: rabbit anti-HA(CST, 3724; WB, 1:5,000), mouse anti-FLAG (Sigma, F3165; WB, 1:5,000), mouse anti-β-tubulin (ZENBIO, 200608; WB, 1:5,000), mouse anti-Myc (CST, 2276; WB, 1:5,000; IF, 1:500), goat anti-IL1β (R&D Systems, BAF401; WB, 1:2,000), rabbit anti-IL1β (Abcam, ab9722; WB, 1:5,000), rabbit anti-GSDMD (CST, 39754; WB: 1:2,000), rabbit anti-IL33 (Proteintech, 12372-1-AP; WB, 1:1,000), rabbit anti-V5 (CST, 13202; WB, 1:1,000; IF, 1:500), mouse anti-V5 (CST, 80076; WB, 1:5,000), rabbit anti-TMED10 (Proteintech, 15199-1-AP; WB, 1:3,000), rabbit anti-TMED2 (Proteintech, 11981-1-AP; WB, 1:3,000), mouse anti-GST (CST, 2624; WB, 1:5,000), rabbit anti-ERGIC53 (Sigma, E1031; WB, 1:2,000), rabbit anti-GFP (CST, 2956; WB, 1:5,000), rabbit anti-Caspase3 (CST, 9662; WB: 1:2,000), rabbit anti-RPN1 (raised against C-terminal peptides corresponding to human protein residues 588–605 and mouse protein residues 576–605; from R. Schekman; WB, 1:5,000) and rabbit anti-E-SARS2 (raised against C-terminal peptides corresponding to the last 25 residues of E-SARS2 protein; purified by ABclonal; WB, 1:1,000). .. Goat anti-rabbit IgG Alexa Fluor 568 (Invitrogen, A-11011), goat anti-mouse IgG Alexa Fluor 568 (Invitrogen, A-11004) and goat anti-mouse IgG Alexa Fluor Plus 647 (Invitrogen, A32733) were used at a dilution of 1:500 for IF.



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    A) Schematic of the experimental setup and samples included in the analysis. B) A total of 8,443 EPCAM+ cells were computationally isolated and cellular transcriptome heterogeneity was visualized using a tSNE plot revealing 12 clusters of cells. C) Left panel: boxplots (interquartile range with minimum and maximum, outliers removed from plot) show expression level distributions of canonical cell type markers in each cluster. Right panel: heatmap shows z-transformed cluster average expression levels of top 50 cluster markers ranked by log-transformed fold change in expression levels in each cluster compared to other clusters in any of the clusters. Clusters were identified by expression of markers canonically associated with cell populations based on published literature, or given a new name (e.g. secretory progenitor, bud tip adjacent). A list of the upregulated genes in the bud tip progenitor cluster (BP) and the basal cell cluster (BC) with top fold change in expression levels relative to all other clusters is shown and provides a gene signature for these cell populations. Cell cluster names and colors correspond to those in Figure 1B. D) Feature plots of canonical bud tip progenitor markers SFTPC and AGER, a canonical marker of alveolar epithelial type 1 cells. E) Protein staining for SOX9 (green), SFTPC (pink), and DAPI (blue) in bud tips of a 16 week fetal lung specimen. Protein staining for SOX9 (green), PDPN (pink), RAGE (also known as AGER; white), and DAPI (blue) in cells adjacent to the bud tips in a 16 week fetal lung sample. Scale bars represent 50 μm. F) Feature plots showing expression of canonical basal cell markers TP63 and KRT5, as well as KRT15 and <t>IL33,</t> which are highly specific to cluster 7. G) Protein staining of tracheal TP63+ basal cells in 17 week fetal lungs for other basal cell markers identified in cluster 7 by scRNA-seq: KRT15 (pink), EGFR (pink), IL33 (pink), F3 (white) and PDPN (pink).
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    Image Search Results


    A) Schematic of the experimental setup and samples included in the analysis. B) A total of 8,443 EPCAM+ cells were computationally isolated and cellular transcriptome heterogeneity was visualized using a tSNE plot revealing 12 clusters of cells. C) Left panel: boxplots (interquartile range with minimum and maximum, outliers removed from plot) show expression level distributions of canonical cell type markers in each cluster. Right panel: heatmap shows z-transformed cluster average expression levels of top 50 cluster markers ranked by log-transformed fold change in expression levels in each cluster compared to other clusters in any of the clusters. Clusters were identified by expression of markers canonically associated with cell populations based on published literature, or given a new name (e.g. secretory progenitor, bud tip adjacent). A list of the upregulated genes in the bud tip progenitor cluster (BP) and the basal cell cluster (BC) with top fold change in expression levels relative to all other clusters is shown and provides a gene signature for these cell populations. Cell cluster names and colors correspond to those in Figure 1B. D) Feature plots of canonical bud tip progenitor markers SFTPC and AGER, a canonical marker of alveolar epithelial type 1 cells. E) Protein staining for SOX9 (green), SFTPC (pink), and DAPI (blue) in bud tips of a 16 week fetal lung specimen. Protein staining for SOX9 (green), PDPN (pink), RAGE (also known as AGER; white), and DAPI (blue) in cells adjacent to the bud tips in a 16 week fetal lung sample. Scale bars represent 50 μm. F) Feature plots showing expression of canonical basal cell markers TP63 and KRT5, as well as KRT15 and IL33, which are highly specific to cluster 7. G) Protein staining of tracheal TP63+ basal cells in 17 week fetal lungs for other basal cell markers identified in cluster 7 by scRNA-seq: KRT15 (pink), EGFR (pink), IL33 (pink), F3 (white) and PDPN (pink).

    Journal: Developmental cell

    Article Title: In vitro and in vivo development of the human airway at single cell resolution

    doi: 10.1016/j.devcel.2020.01.033

    Figure Lengend Snippet: A) Schematic of the experimental setup and samples included in the analysis. B) A total of 8,443 EPCAM+ cells were computationally isolated and cellular transcriptome heterogeneity was visualized using a tSNE plot revealing 12 clusters of cells. C) Left panel: boxplots (interquartile range with minimum and maximum, outliers removed from plot) show expression level distributions of canonical cell type markers in each cluster. Right panel: heatmap shows z-transformed cluster average expression levels of top 50 cluster markers ranked by log-transformed fold change in expression levels in each cluster compared to other clusters in any of the clusters. Clusters were identified by expression of markers canonically associated with cell populations based on published literature, or given a new name (e.g. secretory progenitor, bud tip adjacent). A list of the upregulated genes in the bud tip progenitor cluster (BP) and the basal cell cluster (BC) with top fold change in expression levels relative to all other clusters is shown and provides a gene signature for these cell populations. Cell cluster names and colors correspond to those in Figure 1B. D) Feature plots of canonical bud tip progenitor markers SFTPC and AGER, a canonical marker of alveolar epithelial type 1 cells. E) Protein staining for SOX9 (green), SFTPC (pink), and DAPI (blue) in bud tips of a 16 week fetal lung specimen. Protein staining for SOX9 (green), PDPN (pink), RAGE (also known as AGER; white), and DAPI (blue) in cells adjacent to the bud tips in a 16 week fetal lung sample. Scale bars represent 50 μm. F) Feature plots showing expression of canonical basal cell markers TP63 and KRT5, as well as KRT15 and IL33, which are highly specific to cluster 7. G) Protein staining of tracheal TP63+ basal cells in 17 week fetal lungs for other basal cell markers identified in cluster 7 by scRNA-seq: KRT15 (pink), EGFR (pink), IL33 (pink), F3 (white) and PDPN (pink).

    Article Snippet: Rabbit anti-IL33 , Sigma-Aldrich Atlas Antibodies , HPA024426.

    Techniques: Isolation, Expressing, Transformation Assay, Marker, Staining

    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: In vitro and in vivo development of the human airway at single cell resolution

    doi: 10.1016/j.devcel.2020.01.033

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit anti-IL33 , Sigma-Aldrich Atlas Antibodies , HPA024426.

    Techniques: Transduction, Plasmid Preparation, Recombinant, Multiplex Assay, In Situ Hybridization, Generated, Software